Elimination of pleuropneumonia-like organisms from tissue culture

Elimination of pleuropneumonia-like organisms from tissue culture.

Pleuropneumonia-like organisms (PPLO) are the crabgrass of tissue culture. Many reports exist in the literature of successful attempts to eliminate these organisms from tissue cultures, but usually such reports were stimulated by the failure of some previously described method to be successful in the hands of the investigator. Although our cultures exhibited no overt manifestations of contamina...

Culture of Human Genital "t-strain" Pleuropneumonia-like Organisms.

Ford, Denys K. (University of British Columbia, Vancouver, Canada). Culture of human genital "T-strain" pleuropneumonia-like organisms. J. Bacteriol. 84:1028-1034. 1962.-The conditions under which "T-strain" pleuropneumonia-like organisms, as described by Shepard, are best cultured were investigated. The organisms were found to grow on several types of nutrient agar and broth, of which PPLO med...

[Contamination of tissue cultures with pleuropneumonia-like organisms].

Pleuropneumonia-Like Organisms (PPLO) Mycoplasmataceae

This small book is an attempt to review present knowledge on the morphology, immunology, and pathogenicity of the pleuropneumonia-like organisms (PPLO). The author is a recognized authority on these organisms and the material is presented from her point of view, although she readily admits that there are some who may be in disagreement with her conclusions. Four of the eleven chapters are devot...

Genital strains of human pleuropneumonia-like organisms.

Klieneberger-Nobel (1961) reviewed in detail the significance of large-colony pleuropneumonia-like organisms (PPLO) in the human genital tract. Unfortunately, at that time, the existence of T-strain PPLO, as described by Shepard (1956), was under dispute. Subsequently, however, Shephard's observation that T-strain PPLO could be isolated from the majority of patients with non-gonococcal urethrit...